[Functional Genomics Center Seminar]
     
       
    ▶Subject: Genome-scale Hypothesis Scanning for Genome Science
    
    ▶Speaker: Byung-Kwan Cho , Ph.D. (KAIST)
            
    ▶Date: 4:00PM/Apr./21(Tue.)/2015
           
    ▶Place: Life Science Bldg. #104

    *Abctract
          Over the past decade or so, dramatic developments in our ability to experimentally determine the contents and functions of genomes have taken place. In particular, high-throughput sequencing technologies are now inspiring a new understanding of the genomes on a global scale. In particular, bacterial genomes are organized by structural and functional elements, including promoters, transcription start and termination sites, open reading frames, regulatory noncoding regions, untranslated regions and transcription units. Thus, identification of those genomic elements is prerequisite for understanding the complete regulatory network of a bacterial cell. Here, we measured the frequency of actual members of a heterogeneous transposon mutant pool to determine the contribution of every essential and non-essential element in the E. coli genome under a given growth condition. Also, we applied de novo genome and transcriptome assembly approaches to discover useful gene parts. De novo transcriptome assembly and differential expression analysis based upon NGS provides deeper insights into bacterial cells by metabolic pathway reconstruction without an annotated reference genome sequences. Lastly, we disclosed the dynamics of transcription using ChIP-exo data with extremely high resolution of single base, together with ChIP-seq and RNA-seq data. The mobility of RNAP holoenzyme at transcription initiation was elucidated through genome-scale scanning for association of RNAP and sigma factor with genome. The results support a reliable window for observing and interpreting intricate transcription events in a genomic scale perspective.

    ▶Inquiry: Prof. Inhwan Hwang (279-2128)
          
          * This seminar will be given in English.
   please refrain from taking photos during seminars. *