[2014 Fall Life Sciences & IBB Regular Seminar]
      
        
     ▶Subject: Visualizing biological phenomena by fluorescent molecular spies
     
     ▶Speaker: Sang-Yeob Kim, Ph.D. (Biomedical Research Center, Asan Institute for Life Sciences, ASAN Medical Center)
             
     ▶Date: 4:00PM/Nov./7(Fri)/2014
            
     ▶Place: Auditorium(1F), POSTECH Biotech Center
            
             *Abctract
          After identifying the primary sequence of every protein, biologically relevant protein-protein interactions have been elucidated. The direct visualization of biological function in specific space and time is important for complete understanding of dynamic protein interactions. Light microscopy and fluorescent protein (FP) enable the live cell imaging of the molecular behaviors. However, because of the limit of the resolution, conventional light microscopy can not reveal the physical interactions between protein's partners involved in many cellular processes. To overcome this problem, fluorescent resonance energy transfer (FRET) between a fluorescent donor and an acceptor molecule has been widely used to determine distances in proteins and thus to obtain structural information. In addition, FRET methods have a distinct advantage for applications requiring time-lapse or three-dimensional FRET imaging in living cell and for monitoring for spatio-temporal information of intracellular signaling by using various useful FRET indicators. Here, we visualized biological phenomena by using FRET imaging techniques during phagocytosis. This functional analysis based on FRET in living cell enables us to better understanding of the dynamic linkage of several molecules related in the signal transduction during phagocytosis.

    ▶Inquiry: Prof.Kwan Yong Choi (279-2295)
           
       * This seminar will be given in English