[BK21 Plus Seminar] ▶Subject: 바이오마커 연구의 유용한 도구 Molecular Proteomics: 기술개발과 응용 ▶Speaker: Cheolju Lee, PH.D. (한국과학기술연구원 의공학연구소 테라그노시스연구단) ▶Date: 4:30 PM/Feb. 26(Fri.)/2016 ▶Place: Life Science Bldg. #104 *Abctract In order to discover serological colorectal cancer (CRC) markers, we analyzed cell line secretome to gather proteins of higher potential to be secreted from tissues into circulation. A total of 898 human proteins were identified, of which 62.2% were predicted to be released or shed from cells. The identified proteins were compared with tissue proteomes to find candidate proteins whose expressions were elevated in tumor tissues compared with normal tissues as revealed by (i) quantitative proteomic analysis based on cICAT and mTRAQ or (ii) data mining of immunohistochemical images piled in Human Protein Atlas database. We found that melanotransferrin (TRFM) at the plasma level of CRC patients increased in a comparison with healthy control. Unlike CEA and PAI-1, up-regulation of TRFM in pathological stages I & II groups compared with stages III & IV groups lead us to expect the use TRFM for early-stage diagnosis of CRC. In the presentation, I would like to show how we develop m-TRAQ based quantitative proteomic method, the usefulness of composite human-FBS database in analysis of human secreted proteins, and other researches being performed in our laboratory. ▶Inquiry: Prof. Hwang, Cheol-Sang (279-2352) * This seminar will be given in Korean. please refrain from taking photos during seminars. *