[2015 Fall Life Sciences & IBB Regular Seminar]
            
               
           ▶Subject: Global genome and proteome analyses of skeletal muscle aging
             
           ▶Speaker: Prof. Ki-Sun Kwon (KRIBB)

           ▶Date: 4:00PM/Oct./30(Fri.)/2015
              
           ▶Place: Auditorium(1F), Postech Biotech Center
             
                   *Abctract
                 Aging in muscle is associated with a decrease in mass and strength. Human loses up to 40% muscle at the age of 65. To fully characterize molecular network of aging in skeletal muscle, we performed next-generation sequencing to assess genome-wide expression profiling of microRNA and mRNA as well as DNA methylation, using mouse gastrocnemius muscle at two different ages (6-month-old versus 24-month-old). We found 34 age-regulated miRNAs and 16 novel miRNAs in muscle. Genes regulated either by epigenetic or other mechanism contribute on increase of inflammatory response, stress response, and growth inhibition, while decreasing energy metabolism and muscle development. Age-associated miRNAs might contribute to aging through the control of glucose metabolism, ion homeostasis, and transcription regulation. We also profiled proteomic changes during muscle aging. We found several protein markers useful for diagnostics and characterized the plausible deteriorate mechanisms by the proteins. All these events drive decrease in myoblast differentiation and increase in atrophy, leading to muscle aging. Further, we are going to confirm the candidate genes and networks in the physiological levels. Also we are now screening drug candidates that enhance muscle regeneration by using myoblast differentiation assay. We have selected several positive candidates in animal test and plan to do clinical study to overcome muscle aging.

                 
          ▶Inquiry: Prof. Seung-Jae Lee (279-2351) 
      
             * This seminar will be given in English.
         please refrain from taking photos during seminars. *