[2015 Spring Life Sciences & IBB Regular Seminar]
     
       
   ▶Subject: About the multifunctional roles of the Petunia strigolactone transporter PaPDR1
     
   ▶Speaker: Prof. Enrico Martinoia (Institute of Plant Biology, University of Z?rich)
             

   ▶Date: 4:00PM/April/9(Thur.)/2015
      
   ▶Place: Auditorium(1F), Postech Biotech Center
     
           *Abctract
          Strigolactones (SL), firstly discovered as germination stimulants for parasitic weeds, are carotenoid-derived phytohormones that play major roles in inhibiting lateral bud outgrowth and promoting plant-mycorrhizal symbiosis. Recently, we discovered a strigolactone transporter from Petunia, Petunia axilaris PLEIOTROIC DRUG RESISTANCE 1 (PaPDR1), which is required for efficient mycorrhizal colonization and inhibition of lateral bud outgrowth. While strigolactone release into the medium could be shown, how strigolactones are transported through the plant remained unknown. To answer this question we produced plants expressing a PaPDR1-GFP construct and determined its localization. PaPDR1 exhibits a cell-type, specific asymmetric localization in different root tissues. In root tips, PaPDR1 is co-expressed with the strigolactone biosynthetic gene DAD1 (CCD8) and it is localized at the apical membrane of root hypodermal cells, presumably mediating the shootward transport of strigolactone. Above the root tip, in the hypodermal passage cells (HPCs) that form gates for the entry of mycorrhizal fungi, PaPDR1 is present in the outer-lateral membrane, compatible with its postulated function as strigolactone exporter from root to soil. Transport studies using radiolabeled strigolactone support our localization studies, since a papdr1 mutant displays impaired transport of strigolactones out of the root tip to the shoot as well as into the rhizosphere. Furthermore, we produced plants overexpressing PaPDR1 and could show, that by doing so, strigolactone homeostasis was disturbed and several strigolactone-related phenotypes could be observed.

   ▶Inquiry: Prof.Lee, Youngsook(279-2296)
           
     
     * This seminar will be given in English.
 please refrain from taking photos during seminars. *