▶Subject: Genome research using Next-Generation Sequencing

(Tiger, Whale, Cloned dog, and Cancer genomes)

 ▶Speaker: 조윤성 박사 (Theragen)

 

▶Date: 4:00PM/February/13(Thu)/2014

 

▶Place: Life Science Bldg. #104

  *Abctract
Whole genome data can dramatically enhance our understanding of evolution and adaptation. Also, Next-generation sequencing (NGS) has led researchers to detect disease-associated variations and helped uncover the underlying mechanisms of disease development. In this seminar, we introduce several genome studies using NGS technology.
 
1) Tigers and their close relatives in the genus Panthera are and their wild populations have plummeted during the last century. A de novo assembled whole genome sequence of an Amur tiger was constructed and compared to genome sequences from white tiger, African lion, white African lion, and snow leopard. Compared to the domestic cat reference and other big cat genomes, we found genetic signatures that reflect molecular adaptations consistent with the big cats’ adaptations.
2) The shift from terrestrial to fully aquatic life by whales represents a dramatic evolutionary event in the mammalian lineage. We present a de novo assembly of the minke whale genome, with additional genomic sequences of three minke whales, a fin whale, a bottlenose dolphin, and a finless porpoise, to improve our understanding of the evolutionary changes necessary to survive in an aquatic environment.
3) Cloning is a process that produces genetically identical organisms. However, the genomic degree of genetic resemblance in clones needs to be determined. In this report, the genome of a cloned dog and its donor was compared. Compared with a human monozygotic twin, the genome of the cloned dog showed little difference from the genome of the nuclear donor dog in terms of single nucleotide variations, chromosomal instability, and telomere lengths.
4) Stomach cancer is the third deadliest among all the cancers worldwide. Although incidence of the intestinal-type gastric cancer has decreased, the diffuse-type’s incidence is still increasing and its progression is notoriously aggressive. We analyzed whole genomes and corresponding exomes of diffuse-type gastric cancer, using matched tumor and normal samples.

 ▶Prof.  Inhwan Hwang (279-2128)