[2014 Spring Life Sciences & IBB Regular Seminar] 

 

 

▶Subject: Genome Engineering in Cultured Cells and Animals with Programmable Nucleases
 

▶Speaker: Jin-Soo Kim, Ph.D. (Seoul National University)
 
 
▶Date: 4:00PM/March/21(Fri)/2014
 
  
▶Place: Auditorium(1F), Postech Biotech Center
 

*Abctract
Genome engineering that allows targeted mutagenesis in higher eukaryotic cells and organisms is broadly useful in biology, biotechnology, and medicine. We have developed zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and Cas9 RNA-guided engineered nucleases (RGENs), derived from CRISPR/Cas, to cleave chromosomal DNA in a targeted manner, producing DNA double-strand breaks in cells, the repair of which via endogenous systems gives rise to targeted genome modifications. Cas9 is a sequence-specific endonuclease in type II CRISPR/Cas systems, which confer prokaryotes with adaptive immunity against invading phages and plasmids. Cas9, when complexed with small guide RNAs, recognizes and cleaves target DNA sequences complementary to the guide RNAs in vivo, inducing targeted genome modifications at high frequencies in cultured cells and animals. Unlike ZFNs and TALENs, RGENs can be customized without any cloning step, making them a broadly useful, scalable and expeditious platform for genome editing.

▶Inquiry: Prof. Hwang, Il-Doo (279-2291)

 

* This seminar will be given in English